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Bcl-2 antibody [100/D5]

Anti-Bcl-2 antibody [100/D5] used in IHC (Paraffin sections) (IHC-P). GTX34425
Anti-Bcl-2 antibody [100/D5] used in Western Blot (WB). GTX34425
Anti-Bcl-2 antibody [100/D5] used in Flow cytometry (FACS). GTX34425
Anti-Bcl-2 antibody [100/D5] used in Western Blot (WB). GTX34425

Cat No. GTX34425

Host

Mouse

Clonality

Monoclonal

Clone Name

100/D5

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2μg/ml
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Specificity/Sensitivity

This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein.
We do not recommend use of this product for Mouse,Rat.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide, aa41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein.

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

BCL2 , BCL2, apoptosis regulator , Bcl2 , PPP1R50 , Bcl-2 , BCL2 apoptosis regulator

Cellular Localization

Mitochondrion outer membrane,Nucleus membrane,Endoplasmic reticulum membrane

Background

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

Database

Research Area

DATA IMAGES

Anti-Bcl-2 antibody [100/D5] used in IHC (Paraffin sections) (IHC-P). GTX34425

GTX34425 IHC-P Image

IHC-P analysis of human non-Hodgkin's lymphoma tissue using GTX34425 Bcl-2 antibody [100/D5].

Anti-Bcl-2 antibody [100/D5] used in Western Blot (WB). GTX34425

GTX34425 WB Image

WB analysis of human skin tissue lysate using GTX34425 Bcl-2 antibody [100/D5].

Anti-Bcl-2 antibody [100/D5] used in Flow cytometry (FACS). GTX34425

GTX34425 FACS Image

FACS analysis of Jurkat cells using GTX34425 Bcl-2 antibody [100/D5].
Red : Primary antibody
Green : Isotype control

Anti-Bcl-2 antibody [100/D5] used in Western Blot (WB). GTX34425

GTX34425 WB Image

WB analysis of MCF-7 cell lysate using GTX34425 Bcl-2 antibody [100/D5].

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf