GeneTex
United States (US)

Bcl-2 antibody [100/D5]

GTX34425_20170712_FACS_18121410_363.jpg
GTX34425_20170712_IHC-P_18121410_998.jpg
GTX34425_20170712_WB_18121410_386.jpg

Cat No. GTX34425

Host Mouse
Clonality Monoclonal
Clone Name 100/D5
Isotype IgG1
Application WB, IHC-P, FACS
Reactivity Human
APPLICATION

Application Note

IHC-P:0.5-1ug/ml for 30 minutes at RT, FACS:0.5-1ug/million cells in 0.1ml, WB:0.5-1ug/ml.

Calculated MW

26 kDa. ( Note )

Specificity/Sensitivity

This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 α oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 α oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 α protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

A synthetic peptide, aa41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein.

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Bcl2, Apoptosis Regulator,Bcl-2,Ppp1R50,Bcl2

Background

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX34425_20170712_FACS_18121410_363.jpg

GTX34425 FACS Image

Intracellular staining of Bcl-2 in Jurkat cells using Bcl-2 Monoclonal Antibody (100/D5) (red) and isotype control (green).

GTX34425_20170712_IHC-P_18121410_998.jpg

GTX34425 IHC-P Image

Formalin-fixed, paraffin-embedded human non-Hodgkin's Lymphoma stained with Bcl-2 Monoclonal Antibody (100/D5). Note nuclear membrane & cytoplasmic staining.

GTX34425_20170712_WB_18121410_386.jpg

GTX34425 WB Image

Western Blot of Bcl-2 in human Skin using Bcl-2 Monoclonal Antibody (100/D5).

REFERENCE