GeneTex
United States (US)

Bcl-2 antibody [100]

GTX31237_138_WB_18121410_298.jpg
GTX31237_137_IHC-P_18121410_460.jpg

Cat No. GTX31237

Host Mouse
Clonality Monoclonal
Clone Name 100
Isotype IgG1
Application WB, IHC-P, IHC-Fr, FACS, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IHC-P 1/20-1/40
IHC-Fr 1/20-1/40
FACS 1/50-1/100
IP Assay dependent

Note :

IHC-P
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

FACS
Membrane permeabilisation is required for this application.

Not tested in other applications.

Calculated MW

26 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide, amino acids 41-54 of the Bcl-2 protein

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Bcl2, Apoptosis Regulator,Bcl-2,Ppp1R50,Bcl2

Background

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX31237_138_WB_18121410_298.jpg

GTX31237 WB Image

WB analysis of Jurkat cell lysate using GTX31237 Bcl-2 antibody [100].

GTX31237_137_IHC-P_18121410_460.jpg

GTX31237 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX31237 Bcl-2 antibody [100].