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Bcl-2 antibody [100]

Anti-Bcl-2 antibody [100] used in Western Blot (WB). GTX31237
Anti-Bcl-2 antibody [100] used in IHC (Paraffin sections) (IHC-P). GTX31237

Cat. No. GTX31237

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 100

Isotype

 IgG1

Application

 WB, IHC-P, IHC-Fr, FACS, IP

Reactivity

 Human
Package
100 μg ($399)
See all Bcl-2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P 1/20-1/40
IHC-Fr 1/20-1/40
FACS 1/50-1/100
IP Assay dependent

Note :

IHC-P
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

FACS
Membrane permeabilisation is required for this application.
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide, amino acids 41-54 of the Bcl-2 protein

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCL2 apoptosis regulator , Bcl-2 , PPP1R50

Cellular Localization

Mitochondrion outer membrane , Endoplasmic reticulum membrane

Background

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

Database

Research Area

DATA IMAGES

Anti-Bcl-2 antibody [100] used in Western Blot (WB). GTX31237

GTX31237 WB Image

WB analysis of Jurkat cell lysate using GTX31237 Bcl-2 antibody [100].

Anti-Bcl-2 antibody [100] used in IHC (Paraffin sections) (IHC-P). GTX31237

GTX31237 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX31237 Bcl-2 antibody [100].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399