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Bcl-2 antibody [8C8]

Anti-Bcl-2 antibody [8C8] used in Western Blot (WB). GTX34427
Anti-Bcl-2 antibody [8C8] used in IHC (Paraffin sections) (IHC-P). GTX34427

Cat No. GTX34427

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 8C8

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Pig, Monkey
Package
100 μg ($429)
See all Bcl-2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1ug/ml
ICC/IF 1-2ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Specificity/Sensitivity

This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 α oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 α oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 α protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.

PROPERTIES

Form

Liquid

Buffer

10mM PBS with 0.05% BSA, 0.05% azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml

Antigen Species

Human

Immunogen

A synthetic peptide, aa41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein.

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Bcl2, Apoptosis Regulator , Bcl-2 , Ppp1R50 , Bcl2

Background

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Bcl-2 antibody [8C8] used in Western Blot (WB). GTX34427

GTX34427 WB Image

Western Blot of Bcl-2 in human A) HeLa, B) A549 and C) MCF7 Lysate using Bcl-2 Monoclonal Antibody (8C8).

Anti-Bcl-2 antibody [8C8] used in IHC (Paraffin sections) (IHC-P). GTX34427

GTX34427 IHC-P Image

Formalin-fixed, paraffin-embedded human tonsil stained with Bcl-2 Monoclonal Antibody (8C8). Note cell membrane and cytoplasmic staining.

REFERENCE

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Package List Price ($)
$ 429