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Bcl-2 antibody [AT1B5]

Anti-Bcl-2 antibody [AT1B5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57574
Anti-Bcl-2 antibody [AT1B5] used in Western Blot (WB). GTX57574
Anti-Bcl-2 antibody [AT1B5] used in Flow cytometry (FACS). GTX57574

Cat. No. GTX57574

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT1B5

Isotype

 IgG2b

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all Bcl-2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:250-1:500
ICC/IF 1:250-1:500
FACS Assay dependent
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT1B5 is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Bcl-4 protein.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCL2 apoptosis regulator , BCL2 , BCL2, apoptosis regulator , Bcl2 , PPP1R50 , Bcl-2

Cellular Localization

Mitochondrion outer membrane,Nucleus membrane,Endoplasmic reticulum membrane

Background

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

Database

Research Area

DATA IMAGES

Anti-Bcl-2 antibody [AT1B5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57574

GTX57574 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX57574 Bcl-2 antibody.
Blue: Hoechst 3342
Red: Primary antibody
Dilution: 1:500

Anti-Bcl-2 antibody [AT1B5] used in Western Blot (WB). GTX57574

GTX57574 WB Image

WB analysis of NIH3T3 whole cell lysate using GTX57574 Bcl-2 antibody.
Loading : 35 μg
Dilution : 1:500

Anti-Bcl-2 antibody [AT1B5] used in Flow cytometry (FACS). GTX57574

GTX57574 FACS Image

FACS analysis of HeLa cells using GTX57574 Bcl-2 antibody.
Cell Number: 1 x 10⁶ cells
Primary antibody: Red line
Antibody amount: 2-5 μg

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399