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Bcl-2 antibody [GT11210]

Anti-Bcl-2 antibody [GT11210] used in Western Blot (WB). GTX628905
Anti-Bcl-2 antibody [GT11210] used in IHC (Paraffin sections) (IHC-P). GTX628905

Cat. No. GTX628905

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT11210

Isotype

 IgG2b

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Bcl-2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

human BCL2-transfected 293T cells

Predict Reactivity

Mouse, Rat, Bovine, Cat, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Bcl-2. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888080

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCL2 apoptosis regulator , Bcl-2 , PPP1R50

Cellular Localization

Mitochondrion outer membrane , Nucleus membrane , Endoplasmic reticulum membrane

Background

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Bcl-2 antibody [GT11210] used in Western Blot (WB). GTX628905

GTX628905 WB Image

Bcl-2 antibody [GT11210] detects Bcl-2 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human Bcl-2-transfected 293T cells
12 % SDS-PAGE
Bcl-2 antibody [GT11210] (GTX628905) dilution: 1:5000

Anti-Bcl-2 antibody [GT11210] used in IHC (Paraffin sections) (IHC-P). GTX628905

GTX628905 IHC-P Image

Bcl-2 antibody [GT11210] detects Bcl-2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human tonsil.
Bcl-2 stained by Bcl-2 antibody [GT11210] (GTX628905) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations