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Bcl-2 antibody [GT1213]

Anti-Bcl-2 antibody [GT1213] used in Western Blot (WB). GTX01194
Anti-Bcl-2 antibody [GT1213] used in Western Blot (WB). GTX01194
Anti-Bcl-2 antibody [GT1213] used in Immunoprecipitation (IP). GTX01194
Anti-Bcl-2 antibody [GT1213] used in Western Blot (WB). GTX01194
Anti-Bcl-2 antibody [GT1213] used in IHC (Paraffin sections) (IHC-P). GTX01194

Cat No. GTX01194

Host

Rabbit

Clonality

Monoclonal

Clone Name

GT1213

Isotype

IgG

Application

WB, IHC-P, IP

Reactivity

Human, Mouse
Package
100 μl ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (pH7.3), 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human Bcl-2.

Purification


Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

BCL2 apoptosis regulator , BCL2 , BCL2, apoptosis regulator , Bcl2 , PPP1R50 , Bcl-2

Cellular Localization

Mitochondrion outer membrane,Nucleus membrane,Endoplasmic reticulum membrane

Background

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

Database

Research Area

DATA IMAGES

Anti-Bcl-2 antibody [GT1213] used in Western Blot (WB). GTX01194

GTX01194 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [GT1213] (GTX01194) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Bcl-2 antibody [GT1213] used in Western Blot (WB). GTX01194

GTX01194 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [GT1213] (GTX01194) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bcl-2 antibody [GT1213] used in Immunoprecipitation (IP). GTX01194

GTX01194 IP Image

IP analysis of Mouse lung tissue lysate using GTX01194 Bcl-2 antibody [GT1213].
Total protein amount : 200μg
IP antibody : 3μg
Dilution : 1:1000

Anti-Bcl-2 antibody [GT1213] used in Western Blot (WB). GTX01194

GTX01194 WB Image

WB analysis of various samples using GTX01194 Bcl-2 antibody [GT1213].
Dilution : 1:1000
Loading : 25μg

Anti-Bcl-2 antibody [GT1213] used in IHC (Paraffin sections) (IHC-P). GTX01194

GTX01194 IHC-P Image

IHC-P analysis of human placenta tissue section using GTX01194 Bcl-2 antibody [GT1213].
Dilution : 1:100

REFERENCE

There are currently no references for Bcl-2 antibody [GT1213] (GTX01194). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Bcl-2 antibody [GT1213] (GTX01194). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 379