APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/ml |
| 1-2μg/ml |
| 1-2μg/ml for 30 minutes at RT |
| 1-2μg/10⁶ cells |
|
Note :
IHC-P
Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
Product Note
This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein.
We do not recommend use of this product for Mouse,Rat samples.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthetic peptide, aa41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
BCL2 , BCL2, apoptosis regulator , Bcl2 , PPP1R50 , Bcl-2 , BCL2 apoptosis regulator
Cellular Localization
Mitochondrion outer membrane,Nucleus membrane,Endoplasmic reticulum membrane
Background
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
Database
Research Area
DATA IMAGES
![Anti-Bcl-2 antibody [SPM530] used in IHC (Paraffin sections) (IHC-P). GTX34428](/templates/default/js/lightbox/images/loading.gif "Anti-Bcl-2 antibody [SPM530] used in IHC (Paraffin sections) (IHC-P). GTX34428")
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GTX34428 IHC-P Image
IHC-P analysis of human tonsil tissue using GTX34428 Bcl-2 antibody [SPM530].
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GTX34428 WB Image
WB analysis of MCF-7 cell lysate using GTX34428 Bcl-2 antibody [SPM530].
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REFERENCE
There are currently no references for Bcl-2 antibody [SPM530] (GTX34428). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Bcl-2 antibody [SPM530] (GTX34428). Be the first to share your experience with this product.
![Anti-Bcl-2 antibody [SPM530] used in IHC (Paraffin sections) (IHC-P). GTX34428](/upload/website/prouct_img/small/GTX34428/GTX34428_IHC-P_1_w_23060801_167.webp "Anti-Bcl-2 antibody [SPM530] used in IHC (Paraffin sections) (IHC-P). GTX34428")
![Anti-Bcl-2 antibody [SPM530] used in Western Blot (WB). GTX34428](/upload/website/prouct_img/small/GTX34428/GTX34428_20200115_WB_1533_w_23060801_162.webp "Anti-Bcl-2 antibody [SPM530] used in Western Blot (WB). GTX34428")
![Anti-Bcl-2 antibody [SPM530] used in IHC (Paraffin sections) (IHC-P). GTX34428](/upload/website/prouct_img/normal/GTX34428/GTX34428_IHC-P_1_w_23060801_167.webp "Anti-Bcl-2 antibody [SPM530] used in IHC (Paraffin sections) (IHC-P). GTX34428")
![Anti-Bcl-2 antibody [SPM530] used in Western Blot (WB). GTX34428](/upload/website/prouct_img/normal/GTX34428/GTX34428_20200115_WB_1533_w_23060801_162.webp "Anti-Bcl-2 antibody [SPM530] used in Western Blot (WB). GTX34428")