GeneTex
United States (US)

Bcl-2 antibody

Overexpression
GTX100440_40989_20181126_WB_B_19011519_325.jpg
GTX100440_40989_WB_19011506_495.jpg
GTX100440_40989_WB_B_19011506_845.jpg

Cat No. GTX100440

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

Raji , BCL2 transfected 293T lysate
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Bcl-2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Bcl2, Apoptosis Regulator,Bcl-2,Ppp1R50,Bcl2

Cellular Localization

Mitochondrion outer membrane , Nucleus membrane , Endoplasmic reticulum membrane

Background

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100440_40989_20181126_WB_B_19011519_325.jpg

GTX100440 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody (GTX100440) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100440_40989_WB_19011506_495.jpg

GTX100440 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
12% SDS PAGE
GTX100440 diluted at 1:1000

GTX100440_40989_WB_B_19011506_845.jpg

GTX100440 WB Image

Overexpression

Sample (30 ug of whole cell lysate)
A: Non-transfected 293T lysates
B: BCL2 transfected 293T lysate
12% SDS PAGE
GTX100440 diluted at 1:5000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf