APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/mL |
| 2 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
A20 Cell Lysate(GTX27180) , Human Kidney Tissue Lysate(GTX29995) , Human Kidney Tissue Slide
Predict Reactivity
Rat, Bovine, Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Bcl-2 antibody was raised against a peptide corresponding to 15 amino acids near the N terminus of human Bcl-2.The immunogen is located within the first 50 amino acids of Bcl-2.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
BCL2 apoptosis regulator , Bcl-2 , PPP1R50
Cellular Localization
Mitochondrion outer membrane,Endoplasmic reticulum membrane
Background
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX31686 WB Image
WB analysis of MCF-7 and THP-1 cell lysate using GTX31686 Bcl-2 antibody.
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GTX31686 WB Image
WB analysis of A-20 cell lysate using GTX31686 Bcl-2 antibody.
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GTX31686 IHC-P Image
IHC-P analysis of 4% PFA-fixed Mouse Brain Tissue using GTX31686 Bcl-2 antibody.
Dilution : 20μg/mL
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GTX31686 IHC-P Image
IHC-P analysis of 4% PFA-fixed Human Kidney Cells using GTX31686 Bcl-2 antibody.
Dilution : 10μg/mL
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GTX31686 WB Image
WB analysis of various cell lines using GTX31686 Bcl-2 antibody.
Dilution : 2μg/mL
Loading : 15μg of lysates per lane
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GTX31686 IHC-P Image
IHC-P analysis of formaldehyde fixed Human Kidney Tissue using GTX31686 Bcl-2 antibody.
Antigen retreival : Heat mediation with a citrate buffer (pH6)
Dilution : 2μg/mL
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GTX31686 IHC-P Image
IHC-P analysis of formaldehyde fixed Mouse Brain Tissue using GTX31686 Bcl-2 antibody.
Antigen retreival : Heat mediation with a citrate buffer (pH6)
Dilution : 5μg/mL
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REFERENCE
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REVIEW
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