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Bcl-2 antibody

Anti-Bcl-2 antibody used in IHC (Paraffin sections) (IHC-P). GTX31686
Anti-Bcl-2 antibody used in Western Blot (WB). GTX31686
Anti-Bcl-2 antibody used in IHC (Paraffin sections) (IHC-P). GTX31686
Anti-Bcl-2 antibody used in IHC (Paraffin sections) (IHC-P). GTX31686
Anti-Bcl-2 antibody used in Western Blot (WB). GTX31686
Anti-Bcl-2 antibody used in IHC (Paraffin sections) (IHC-P). GTX31686
Anti-Bcl-2 antibody used in Western Blot (WB). GTX31686

Cat No. GTX31686

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, ELISA

Reactivity

Human, Mouse
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2μg/mL
IHC-P 2 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

A20 Cell Lysate(GTX27180) , Human Kidney Tissue Lysate(GTX29995) , Human Kidney Tissue Slide

Predict Reactivity

Rat, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Bcl-2 antibody was raised against a peptide corresponding to 15 amino acids near the N terminus of human Bcl-2.The immunogen is located within the first 50 amino acids of Bcl-2.

Purification

Purified by ion exchange chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

BCL2 apoptosis regulator , Bcl-2 , PPP1R50

Cellular Localization

Mitochondrion outer membrane,Endoplasmic reticulum membrane

Background

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Bcl-2 antibody used in IHC (Paraffin sections) (IHC-P). GTX31686

GTX31686 IHC-P Image

IHC-P analysis of 4% PFA-fixed Human Kidney Cells using GTX31686 Bcl-2 antibody.
Dilution : 10μg/mL

Anti-Bcl-2 antibody used in Western Blot (WB). GTX31686

GTX31686 WB Image

WB analysis of A-20 cell lysate using GTX31686 Bcl-2 antibody.

Anti-Bcl-2 antibody used in IHC (Paraffin sections) (IHC-P). GTX31686

GTX31686 IHC-P Image

IHC-P analysis of 4% PFA-fixed Mouse Brain Tissue using GTX31686 Bcl-2 antibody.
Dilution : 20μg/mL

Anti-Bcl-2 antibody used in IHC (Paraffin sections) (IHC-P). GTX31686

GTX31686 IHC-P Image

IHC-P analysis of formaldehyde fixed Mouse Brain Tissue using GTX31686 Bcl-2 antibody.
Antigen retreival : Heat mediation with a citrate buffer (pH6)
Dilution : 5μg/mL

Anti-Bcl-2 antibody used in Western Blot (WB). GTX31686

GTX31686 WB Image

WB analysis of MCF-7 and THP-1 cell lysate using GTX31686 Bcl-2 antibody.

Anti-Bcl-2 antibody used in IHC (Paraffin sections) (IHC-P). GTX31686

GTX31686 IHC-P Image

IHC-P analysis of formaldehyde fixed Human Kidney Tissue using GTX31686 Bcl-2 antibody.
Antigen retreival : Heat mediation with a citrate buffer (pH6)
Dilution : 2μg/mL

Anti-Bcl-2 antibody used in Western Blot (WB). GTX31686

GTX31686 WB Image

WB analysis of various cell lines using GTX31686 Bcl-2 antibody.
Dilution : 2μg/mL
Loading : 15μg of lysates per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339