GeneTex
United States (US)

Bcl-2 antibody

GTX31687_276_ICC-IF_20180221_18121410_677.jpg
GTX31687_2855_WB_20180221_18121410_606.jpg

Cat No. GTX31687

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 4 μg/mL
ICC/IF 2 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

Daudi Cell Lysate(GTX23951) , A-20 Cell Slide(GTX25541) , A20 Cell Lysate(GTX27180)
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Bcl-2 antibody was raised against a peptide corresponding to 14 amino acids near the middle of human Bcl-2.The immunogen is located within amino acids 80 - 130 of Bcl-2.

Purification

Purified by ion exchange chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Bcl2, Apoptosis Regulator,Bcl-2,Ppp1R50,Bcl2

Background

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX31687_276_ICC-IF_20180221_18121410_677.jpg

GTX31687 ICC/IF Image

ICC/IF analysis of A-20 cells using GTX31687 Bcl-2 antibody.
Working concentration : 2 μg/ml

GTX31687_2855_WB_20180221_18121410_606.jpg

GTX31687 WB Image

WB analysis of (A) Daudi and (B) A-20 cell lysates using GTX31687 Bcl-2 antibody.
Working concentration : 2 μg/ml