APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
Jurkat , Jurkat (1 μM paclitaxel treatment for 16 hr)
Predict Reactivity
Cat(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
2.06 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide corresponding to residues around human Bcl-2 (phospho Ser87). The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886067
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
BCL2 apoptosis regulator , Bcl-2 , PPP1R50
Cellular Localization
Mitochondrion outer membrane , Nucleus membrane , Endoplasmic reticulum membrane
Background
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX129654 WB Image
Untreated (–) and treated (+) Jurkat whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with BCL2 (phospho S87) antibody (GTX129654) diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.
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REFERENCE
There are currently no references for Bcl-2 (phospho Ser87) antibody (GTX129654). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Bcl-2 (phospho Ser87) antibody (GTX129654). Be the first to share your experience with this product.
