GeneTex
United States (US)

Bcl-2 (phospho Ser87) antibody

Anti-Bcl-2 (phospho Ser87) antibody used in Western Blot (WB). GTX32372
Anti-Bcl-2 (phospho Ser87) antibody used in IHC (Paraffin sections) (IHC-P). GTX32372

Cat No. GTX32372

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

26 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of Bcl-2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Bcl2, Apoptosis Regulator,Bcl-2,Ppp1R50,Bcl2

Cellular Localization

Endoplasmic reticulum membrane,Mitochondrion outer membrane,Nucleus membrane

Background

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

Database

Research Area

DATA IMAGES
Anti-Bcl-2 (phospho Ser87) antibody used in Western Blot (WB). GTX32372

GTX32372 WB Image

WB analysis of UV-treated HEK293T (A) whole cell lysates using GTX32372 Bcl-2 (phospho Ser87) antibody.

Anti-Bcl-2 (phospho Ser87) antibody used in IHC (Paraffin sections) (IHC-P). GTX32372

GTX32372 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32372 Bcl-2 (phospho Ser87) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)