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Bcl-2 (phospho Thr56) antibody

Anti-Bcl-2 (phospho Thr56) antibody used in IHC (Paraffin sections) (IHC-P). GTX21908
Anti-Bcl-2 (phospho Thr56) antibody used in Western Blot (WB). GTX21908

Cat No. GTX21908

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
50 μg ($239)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human BCL-2 around the phosphorylation site of threonine 56 (G-H-Tp-P-H).

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

BCL2,BCL2, apoptosis regulator,Bcl2,PPP1R50,Bcl-2,BCL2 apoptosis regulator

Cellular Localization

Mitochondrion outer membrane,Nucleus membrane,Endoplasmic reticulum membrane

Background

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

Database

Research Area

DATA IMAGES

Anti-Bcl-2 (phospho Thr56) antibody used in IHC (Paraffin sections) (IHC-P). GTX21908

GTX21908 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX21908 Bcl-2 (phospho Thr56) antibody.

Anti-Bcl-2 (phospho Thr56) antibody used in Western Blot (WB). GTX21908

GTX21908 WB Image

WB analysis of MDA435 cells treated with sorbtiol (0.4M, 30min) lysate using GTX21908 Bcl-2 (phospho Thr56) antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 239