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Bcl-2 (phospho Thr69) antibody

Anti-Bcl-2 (phospho Thr69) antibody used in Western Blot (WB). GTX55105
Anti-Bcl-2 (phospho Thr69) antibody used in IHC (Paraffin sections) (IHC-P). GTX55105

Cat. No. GTX55105

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Bcl-2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of Bcl-2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCL2 apoptosis regulator , Bcl-2 , PPP1R50

Cellular Localization

Mitochondrion outer membrane,Endoplasmic reticulum membrane

Background

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

Database

Research Area

DATA IMAGES

Anti-Bcl-2 (phospho Thr69) antibody used in Western Blot (WB). GTX55105

GTX55105 WB Image

WB analysis of UV-treated HepG2 (A), HeLa (B), HL60 (C), THP1 (D), paclitaxel-treated Jurkat (E), H2O2-treated NIH3T3(F), H2O2-treated PC12(G) whole cell lysates using GTX55105 Bcl-2 (phospho Thr69) antibody.

Anti-Bcl-2 (phospho Thr69) antibody used in IHC (Paraffin sections) (IHC-P). GTX55105

GTX55105 IHC-P Image

IHC-P analysis of formalin fixed human brain tissue section using GTX55105 Bcl-2 (phospho Thr69) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399