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Bcl-X antibody [016]

Anti-Bcl-X antibody [016] used in Western Blot (WB). GTX02028
Anti-Bcl-X antibody [016] used in Immunoprecipitation (IP). GTX02028

Cat No. GTX02028

Host

Rabbit

Clonality

Monoclonal

Clone Name

016

Isotype

IgG

Application

WB, IP

Reactivity

Human, Mouse
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
IP 0.2-1 μl/mg of lysate
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.2μm-filtered PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant Human BCL2L1 / Bcl-XL protein

Purification

Purified by Protein A

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

BCL2 like 1 , BCL-XL/S , BCL2L , BCLX , Bcl-X , PPP1R52

Cellular Localization

Mitochondrion outer membrane , Mitochondrion matrix , Cytoplasm, cytosol

Background

The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Alternative splicing results in multiple transcript variants encoding two different isoforms. The longer isoform acts as an apoptotic inhibitor and the shorter isoform acts as an apoptotic activator. [provided by RefSeq, Dec 2015]

Database

Research Area

DATA IMAGES

Anti-Bcl-X antibody [016] used in Western Blot (WB). GTX02028

GTX02028 WB Image

WB analysis of various samples using GTX02028 Bcl-X antibody [016].
Lane A : HepG2 whole cell lysate
Lane B : K562 whole cell lysate
Lane C : NIH-3T3 whole cell lysate
Dilution : 1:500
Loading : 30 μg

Anti-Bcl-X antibody [016] used in Immunoprecipitation (IP). GTX02028

GTX02028 IP Image

IP analysis of Jurkat whole cell lysate using GTX02028 Bcl-X antibody [016].
IP antibody : 1 μl per 0.5 mg total whole cell lysate
Dilution : 1:500
50 % Protein G agarose : 15 μl

REFERENCE

There are currently no references for Bcl-X antibody [016] (GTX02028). Be the first to share your publications with this product.

REVIEW

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Package List Price ($)
$ 339