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Bcl-X antibody [7B2.5] (PE)

Orthogonal
Anti-Bcl-X antibody [7B2.5] (PE) used in Flow cytometry (FACS). GTX46035

Cat. No. GTX46035

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 7B2.5

Isotype

 IgG3

Application

 WB, FACS, IP

Reactivity

 Human, Mouse
Package
50 μg ($399)
See all Bcl-X products

APPLICATION

Application Note

Recommended dilutions: FACS: Use 0.3μg for 10^6 cells. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 29 kDa. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

26 kDa. ( Note )

Product Note

GTX46035 recognises Bcl xL.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full length protein: Bcl xL (Human)

Purification

IgG fraction

Conjugation

Phycoerythrin (PE)

RRID

AB_774264

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCL2 like 1 , BCL-XL/S , BCL2L , BCLX , Bcl-X , PPP1R52

Cellular Localization

Mitochondrion outer membrane , Mitochondrion matrix , Cytoplasm, cytosol

Background

The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Alternative splicing results in multiple transcript variants encoding two different isoforms. The longer isoform acts as an apoptotic inhibitor and the shorter isoform acts as an apoptotic activator. [provided by RefSeq, Dec 2015]

Database

Research Area

DATA IMAGES

Anti-Bcl-X antibody [7B2.5] (PE) used in Flow cytometry (FACS). GTX46035

GTX46035 FACS Image

Orthogonal

The data was published in the journal J Exp Clin Cancer Res in 2009. PMID: 19563663

REFERENCE

Application Reference

Badr G et al. Cell Immunol 2010; 263 (1) : 31-40 type i * (ifn-alpha/beta) rescues b-lymphocytes from apoptosis via pi3kdelta/akt, rho-a, nfkappab and bcl-2/bcl(xl).
Nilsonne G et al. J Exp Clin Cancer Res 2009; 28 : 92 Phenotype-dependent apoptosis signalling in mesothelioma cells after selenite exposure.
Application : FACS
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399