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Bcl-X antibody [7B2.5] (PE)

Cat No. GTX46035

Host

Mouse

Clonality

Monoclonal

Clone Name

7B2.5

Isotype

IgG3

Application

WB, FACS, IP

Reactivity

Human, Mouse
Package
50 μg ($379)

APPLICATION

Application Note

Recommended dilutions: FACS: Use 0.3μg for 10^6 cells. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 29 kDa. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

26 kDa. ( Note )

Product Note

GTX46035 recognises Bcl xL.

PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full length protein: Bcl xL (Human)

Purification

IgG fraction

Conjugation

Phycoerythrin (PE)

RRID

AB_774264

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

BCL2 like 1 , BCL-XL/S , BCL2L , BCLX , Bcl-X , PPP1R52

Cellular Localization

Mitochondrion outer membrane , Mitochondrion matrix , Cytoplasm, cytosol

Background

The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Alternative splicing results in multiple transcript variants encoding two different isoforms. The longer isoform acts as an apoptotic inhibitor and the shorter isoform acts as an apoptotic activator. [provided by RefSeq, Dec 2015]

Database

Research Area

REFERENCE

REVIEW

Bcl-X antibody [7B2.5] (PE) Cat No. GTX46035

Rating ( Average 5 based on 1 users reviews)
Application
Flow cytometry(FACS) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : FACS
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Exp Clin Cancer Res in 2009. PMID: 19563663
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 379