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Bcl-XL antibody [4A9]

Anti-Bcl-XL antibody [4A9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84834
Anti-Bcl-XL antibody [4A9] used in Western Blot (WB). GTX84834
Anti-Bcl-XL antibody [4A9] used in IHC (Paraffin sections) (IHC-P). GTX84834
Anti-Bcl-XL antibody [4A9] used in IHC (Paraffin sections) (IHC-P). GTX84834
Anti-Bcl-XL antibody [4A9] used in Western Blot (WB). GTX84834
Anti-Bcl-XL antibody [4A9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84834
Anti-Bcl-XL antibody [4A9] used in Western Blot (WB). GTX84834

Cat No. GTX84834

Host

Mouse

Clonality

Monoclonal

Clone Name

4A9

Isotype

IgG2a

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($459)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:200-1:1000
ICC/IF 1:100
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.25 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment expressed in E.coli corresponding to amino acids 1-212 of human Bcl-XL

Purification

Purified IgG

Conjugation

Unconjugated

RRID

AB_10728062

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

BCL2 like 1 , BCL-XL/S , BCL2L , BCLX , Bcl-X , PPP1R52

Cellular Localization

Mitochondrion outer membrane,Mitochondrion matrix,Cytoplasm, cytosol

Background

Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Isoform Bcl-X(S) promotes apoptosis.

Database

Research Area

DATA IMAGES

Anti-Bcl-XL antibody [4A9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84834

GTX84834 ICC/IF Image

ICC/IF analysis of HT29 cells using GTX84834 Bcl-XL antibody [4A9].

Anti-Bcl-XL antibody [4A9] used in Western Blot (WB). GTX84834

GTX84834 WB Image

WB analysis of various cell lines using GTX84834 Bcl-XL antibody [4A9].
Loading : 10 ug per lane
Dilution : 1:200

Anti-Bcl-XL antibody [4A9] used in IHC (Paraffin sections) (IHC-P). GTX84834

GTX84834 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX84834 Bcl-XL antibody [4A9].

Anti-Bcl-XL antibody [4A9] used in IHC (Paraffin sections) (IHC-P). GTX84834

GTX84834 IHC-P Image

IHC-P analysis of human endometrium adenocarcinoma tissue using GTX84834 Bcl-XL antibody [4A9].

Anti-Bcl-XL antibody [4A9] used in Western Blot (WB). GTX84834

GTX84834 WB Image

WB analysis of various samples using GTX84834 Bcl-XL antibody [4A9].
Loading : 10 ug per lane
Dilution : 1:200

Anti-Bcl-XL antibody [4A9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84834

GTX84834 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with Bcl-XL plasmid using GTX84834 Bcl-XL antibody [4A9].

Anti-Bcl-XL antibody [4A9] used in Western Blot (WB). GTX84834

GTX84834 WB Image

WB analysis of HEK293T cells transfected with Bcl-XL plasmid (Right) or empty vector (Left) for 48 hrs using GTX84834 Bcl-XL antibody [4A9].
Loading : 5 ug per lane

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 459