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Bcl-XL antibody

Anti-Bcl-XL antibody used in Western Blot (WB). GTX59678

Cat. No. GTX59678

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human, Mouse, Rat
Package
100 μg ($419)
See all Bcl-XL products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5-4 μg/ml
IP 20 μg/ml
Not tested in other applications.

Calculated MW

26.05 kDa. ( Note )

Product Note

The antibody detects the 30 kDa Bcl-xL protein. It does not react with other Bcl-2 family members.

PROPERTIES

Form

Liquid

Buffer

PBS pH7.2, 30% Glycerol, 0.5% BSA

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide surrounding amino acid 56 of human Bcl-xL

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_10722492

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCL2 like 1 , BCL2L , BCL2L1 , BCLX , BCLXL/S , BclX , PPP1R52 , Bcl-X

Cellular Localization

Mitochondrion inner membrane,Mitochondrion outer membrane,Mitochondrion matrix,Cytoplasmic vesicle,Secretory vesicle,Synaptic vesicle membrane,Cytoplasm,Cytosol,Cytoplasm,Cytoskeleton,Microtubule organizing center,Centrosome,Nucleus membrane

Background

The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Alternative splicing results in multiple transcript variants encoding two different isoforms. The longer isoform acts as an apoptotic inhibitor and the shorter isoform acts as an apoptotic activator. [provided by RefSeq, Dec 2015]

Database

Research Area

DATA IMAGES

Anti-Bcl-XL antibody used in Western Blot (WB). GTX59678

GTX59678 WB Image

WB analysis of Jurkat (Lane 1 & 2), mouse small intestine (Lane 3), and rat kidney (Lane 4) lysates using GTX59678 Bcl-XL antibody.

REFERENCE

Application Reference

Lee CC et al. PLoS One 2017; 12 (3) : e0173890 the microrna-302b-inhibited *-like growth factor-binding protein 2 signaling pathway induces glioma cell apoptosis by targeting nuclear factor ia.
Application : WB
Reactivity : Human
Hsiao CJ et al. J Nat Prod 2014; 77 (4) : 758-65 Cephalochromin Induces G0/G1 Cell Cycle Arrest and Apoptosis in A549 Human Non-Small-Cell Lung Cancer Cells by Inflicting Mitochondrial Disruption.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 419