GeneTex
United States (US)

Bcl-XS antibody

IP/MS
GTX124266_40744_20160310_WB_R_18121410_254.jpg
GTX124266_40744_IHC_18121410_371.jpg
GTX124266_40744_WB_18121410_229.jpg
GTX124266_40744_IHC_2_18121410_394.jpg

Cat No. GTX124266

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IP
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

18.9 kDa. ( Note )

Positive Control

A549 , H1299 , HCT116 , MCF-7 , PC-12 , Rat2

Specificity/Sensitivity

GTX124266 Bcl-XS antibody was designed specific for BCL-XS
IP/MS validation was supported by references (PMID:30377416)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Bcl-XS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Bcl2 Like 1,Bcl-Xl/S,Bcl2L,Bclx,Bcl-X,Ppp1R52,Bcl2L1

Cellular Localization

Mitochondrion membrane; Single-pass membrane protein , Nucleus membrane; Single-pass membrane protein; Cytoplasmic side , Mitochondrion membrane , Nucleus membrane

Background

The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX124266_40744_20160310_WB_R_18121410_254.jpg

GTX124266 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Bcl-XS antibody (GTX124266) diluted at 1:1000.

GTX124266_40744_IHC_18121410_371.jpg

GTX124266 IHC-P Image

Bcl-XS antibody detects Bcl-XS protein at cytosol on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
Bcl-XS antibody (GTX124266) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX124266_40744_WB_18121410_229.jpg

GTX124266 WB Image

Bcl-XS antibody detects Bcl-XS protein by western blot analysis.
A. 30 μg A549 whole cell lysate/extract
B. 30 μg H1299 whole cell lysate/extract
C. 30 μg HCT116 whole cell lysate/extract
D. 30 μg MCF-7 whole cell lysate/extract
12 % SDS-PAGE
Bcl-XS antibody (GTX124266) dilution: 1:1000

GTX124266_40744_IHC_2_18121410_394.jpg

GTX124266 IHC-P Image

Bcl-XS antibody detects Bcl-XS protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
Bcl-XS antibody (GTX124266) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf