APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
BCL2A1-transfected 293T
Predict Reactivity
Pig, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 1% BSA, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.81 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Bcl2A1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11162727
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
BCL2 related protein A1 , ACC-1 , ACC-2 , ACC1 , ACC2 , BCL2L5 , BFL1 , GRS , HBPA1
Cellular Localization
Cytoplasm
Background
This gene encodes a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators that are involved in a wide variety of cellular activities such as embryonic development, homeostasis and tumorigenesis. The protein encoded by this gene is able to reduce the release of pro-apoptotic cytochrome c from mitochondria and block caspase activation. This gene is a direct transcription target of NF-kappa B in response to inflammatory mediators, and is up-regulated by different extracellular signals, such as granulocyte-macrophage colony-stimulating factor (GM-CSF), CD40, phorbol ester and inflammatory cytokine TNF and IL-1, which suggests a cytoprotective function that is essential for lymphocyte activation as well as cell survival. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-Bcl2A1 antibody [N1C3] used in Western Blot (WB). GTX105641](/templates/default/js/lightbox/images/loading.gif "Anti-Bcl2A1 antibody [N1C3] used in Western Blot (WB). GTX105641")
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GTX105641 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with Bcl2A1 antibody [N1C3] (GTX105641) diluted at 1:500 and competitor's antibody (sc-8351) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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REFERENCE
There are currently no references for Bcl2A1 antibody [N1C3] (GTX105641). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Bcl2A1 antibody [N1C3] (GTX105641). Be the first to share your experience with this product.
![Anti-Bcl2A1 antibody [N1C3] used in Western Blot (WB). GTX105641](/upload/website/prouct_img/small/GTX105641/GTX105641_40541_20171222_WB_B_competitor_watermark_19011506_299.jpg "Anti-Bcl2A1 antibody [N1C3] used in Western Blot (WB). GTX105641")
![Anti-Bcl2A1 antibody [N1C3] used in Western Blot (WB). GTX105641](/upload/website/prouct_img/normal/GTX105641/GTX105641_40541_20171222_WB_B_competitor_watermark_19011506_299.jpg "Anti-Bcl2A1 antibody [N1C3] used in Western Blot (WB). GTX105641")