APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:1000 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Human, Mouse, Rat(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide sequence around phosphorylation site of tyrosine 360 (T-T-Y(p)-R-M) derived from human Bcr.
Purification
Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
BCR activator of RhoGEF and GTPase , ALL , BCR1 , CML , D22S11 , D22S662 , PHL
Background
A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
|
GTX55399 WB Image
WB analysis of extracts from COS-7 tissue using GTX55399 Bcr (phospho Tyr360) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide
|
REFERENCE
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REVIEW
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