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Beclin 1 antibody [2A4]

Anti-Beclin 1 antibody [2A4] used in Western Blot (WB). GTX60413
Anti-Beclin 1 antibody [2A4] used in IHC (Paraffin sections) (IHC-P). GTX60413
Anti-Beclin 1 antibody [2A4] used in Flow cytometry (FACS). GTX60413
Anti-Beclin 1 antibody [2A4] used in ELISA (ELISA). GTX60413

Cat. No. GTX60413

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2A4

Isotype

 IgG1

Application

 WB, IHC-P, FACS, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all Beclin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human BECN1 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_2887964

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

beclin 1 , ATG6 , VPS30 , beclin1

Cellular Localization

Cytoplasm,Endosome membrane,Endoplasmic reticulum membrane,Endosome,Nucleus

Background

Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration (Zhong et al., 2009 [PubMed 19270693]).[supplied by OMIM, Jul 2010]

Database

Research Area

DATA IMAGES

Anti-Beclin 1 antibody [2A4] used in Western Blot (WB). GTX60413

GTX60413 WB Image

WB analysis of HeLa (1), A431 (2), MCF-7 (3), RAJI (4), Jurkat (5) and SKBR-3 (6) cell lysate using GTX60413 Beclin 1 antibody [2A4].

Anti-Beclin 1 antibody [2A4] used in IHC (Paraffin sections) (IHC-P). GTX60413

GTX60413 IHC-P Image

IHC-P analysis of breast cancer tissue (left) and liver cancer tissue (right) using GTX60413 Beclin 1 antibody [2A4].

Anti-Beclin 1 antibody [2A4] used in Flow cytometry (FACS). GTX60413

GTX60413 FACS Image

FACS analysis of RAJI cells using GTX60413 Beclin 1 antibody [2A4].
Green : Beclin 1
Purple : negative control

Anti-Beclin 1 antibody [2A4] used in ELISA (ELISA). GTX60413

GTX60413 ELISA Image

ELISA analysis of antigen using GTX60413 Beclin 1 antibody [2A4].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

REFERENCE

Application Reference

Tsai JH et al. Int J Mol Sci 2016; 17 (8) 1-(2-Hydroxy-5-methylphenyl)-3-phenyl-1,3-propanedione Induces G1 Cell Cycle Arrest and Autophagy in HeLa Cervical Cancer Cells.
Application : WB
Reactivity : Human
Su YC et al. J Tradit Complement Med 2012; 2 (4) : 312-22 Eburicoic Acid, an Active Triterpenoid from the Fruiting Bodies of Basswood Cultivated Antrodia cinnamomea, Induces ER Stress-Mediated Autophagy in Human Hepatoma Cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399