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Beclin 1 antibody [5C2]

Anti-Beclin 1 antibody [5C2] used in IHC (Paraffin sections) (IHC-P). GTX34055
Anti-Beclin 1 antibody [5C2] used in IHC (Paraffin sections) (IHC-P). GTX34055
Anti-Beclin 1 antibody [5C2] used in Western Blot (WB). GTX34055

Cat No. GTX34055

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5C2

Isotype

 IgG1

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($319)
See all Beclin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:2000
IHC-P 1:100-1:200
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% glycerol, 0.5% BSA, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

synthetic peptide derived from Beclin-1 at 110-190 aa.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ATG6,BECN1,VPS30,beclin 1,beclin1,Beclin-1

Cellular Localization

Cytoplasm,Golgi apparatus,Trans-Golgi network membrane,Endosome membrane,Endoplasmic reticulum membrane,Mitochondrion membrane,Endosome,Cytoplasmic vesicle,Autophagosome,Mitochondrion,Nucleus

Background

This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-Beclin 1 antibody [5C2] used in IHC (Paraffin sections) (IHC-P). GTX34055

GTX34055 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX34055 Beclin 1 antibody [5C2].
Dilution : 1:200

Anti-Beclin 1 antibody [5C2] used in IHC (Paraffin sections) (IHC-P). GTX34055

GTX34055 IHC-P Image

IHC-P analysis of human brain tissue using GTX34055 Beclin 1 antibody [5C2].
Dilution : 1:200

Anti-Beclin 1 antibody [5C2] used in Western Blot (WB). GTX34055

GTX34055 WB Image

WB analysis of (1) 293T cell lysate, (2) C2C12 cell lysate, (3) rat brain tissue lysates using GTX34055 Beclin 1 antibody [5C2].
Dilution : 1:2000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 319