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Beclin 1 antibody [AT45E8]

Anti-Beclin 1 antibody [AT45E8] used in Flow cytometry (FACS). GTX57617
Anti-Beclin 1 antibody [AT45E8] used in Western Blot (WB). GTX57617
Anti-Beclin 1 antibody [AT45E8] used in Western Blot (WB). GTX57617
Anti-Beclin 1 antibody [AT45E8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57617

Cat. No. GTX57617

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT45E8

Isotype

 IgG2b

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all Beclin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT45E8 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human BECN3 protein.

Purification

Protein A Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

beclin 1 , ATG6 , BECN1 , VPS30 , beclin1 , Beclin-1

Cellular Localization

Cytoplasm,Golgi apparatus,Trans-Golgi network membrane,Endosome membrane,Endoplasmic reticulum membrane,Mitochondrion membrane,Endosome,Cytoplasmic vesicle,Autophagosome,Mitochondrion,Nucleus

Background

This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-Beclin 1 antibody [AT45E8] used in Flow cytometry (FACS). GTX57617

GTX57617 FACS Image

FACS analysis of HeLa cells using GTX57617 Beclin 1 antibody.
Cell Number: 1 x 10⁶ cells
Red: Primary antibody
Antibody amount: 2-5 μg

Anti-Beclin 1 antibody [AT45E8] used in Western Blot (WB). GTX57617

GTX57617 WB Image

WB analysis of various samples using GTX57617 Beclin 1 antibody.
Lane 1 : 293T cell lysate
Lane 2 : BECN1 transfected 293T cell lysate
Loading : 10 μg
Dilution : 1:1000

Anti-Beclin 1 antibody [AT45E8] used in Western Blot (WB). GTX57617

GTX57617 WB Image

WB analysis of various samples using GTX57617 Beclin 1 antibody.
Lane 1 : Recombinant Human BECN1 protein (10 ng)
Lane 2 : Balb/3T3 whole cell lysate (40 μg)

Anti-Beclin 1 antibody [AT45E8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57617

GTX57617 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX57617 Beclin 1 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399