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Beclin 1 antibody

KO/KD Comparison Overexpression
Anti-Beclin 1 antibody used in Western Blot (WB). GTX133555
Anti-Beclin 1 antibody used in Western Blot (WB). GTX133555
Anti-Beclin 1 antibody used in Western Blot (WB). GTX133555
Anti-Beclin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133555
Anti-Beclin 1 antibody used in Western Blot (WB). GTX133555

Cat. No. GTX133555

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Beclin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

unboiled BECN1-transfected 293T

Predict Reactivity

Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.52 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Beclin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887020

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

beclin 1 , ATG6 , VPS30 , beclin1

Cellular Localization

Cytoplasm , Golgi apparatus membrane; Peripheral membrane protein , Cytoplasm , Golgi apparatus membrane

Background

Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration (Zhong et al., 2009 [PubMed 19270693]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Beclin 1 antibody used in Western Blot (WB). GTX133555

GTX133555 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Beclin 1 antibody (GTX133555) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Beclin 1 antibody used in Western Blot (WB). GTX133555

GTX133555 WB Image

KO/KD

Wild-type (WT) and Beclin 1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Beclin 1 antibody (GTX133555) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Beclin 1 antibody used in Western Blot (WB). GTX133555

GTX133555 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Beclin 1 antibody (GTX133555) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Beclin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133555

GTX133555 IHC-P Image

Beclin 1 antibody detects Beclin 1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Beclin 1 stained by Beclin 1 antibody (GTX133555) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Beclin 1 antibody used in Western Blot (WB). GTX133555

GTX133555 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Beclin 1 antibody (GTX133555) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Dalle Carbonare L et al. Cells 2024; 13 (5) Exploring the Interplay of RUNX2 and CXCR4 in Melanoma Progression.
Lee CW et al. Molecules 2022; 27 (2) : 373 Naringenin Induces ROS-Mediated ER Stress, Autophagy, and Apoptosis in Human Osteosarcoma Cell Lines.
Application : WB
Reactivity : Human
Kuo-Hao Ho et al. Neurotherapeutics 2021; 18 (2) : 1371-1392 Guanabenz Sensitizes Glioblastoma Cells to Sunitinib by Inhibiting GADD34-Mediated Autophagic Signaling.
Application : WB
Reactivity : Human
Huang CY et al. Nutrients 2020; 12 (6) Attenuation of Lipopolysaccharide-Induced Acute Lung Injury by Hispolon in Mice, Through Regulating the TLR4/PI3K/Akt/mTOR and Keap1/Nrf2/HO-1 Pathways, and Suppressing Oxidative Stress-Mediated ER Stress-Induced Apoptosis and Autophagy.
Application : WB
Reactivity : Mouse
Tsai CY et al. Int J Mol Sci 2017; 19 (1) Epigallocatechin-3-Gallate Suppresses Human Herpesvirus 8 Replication and Induces ROS Leading to Apoptosis and Autophagy in Primary Effusion Lymphoma Cells.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169