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Beclin 1 antibody

Anti-Beclin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31722
Anti-Beclin 1 antibody used in Western Blot (WB). GTX31722
Anti-Beclin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31722
Anti-Beclin 1 antibody used in Western Blot (WB). GTX31722
Anti-Beclin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31722
Anti-Beclin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31722
Anti-Beclin 1 antibody used in Western Blot (WB). GTX31722
Anti-Beclin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31722
Anti-Beclin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31722
Anti-Beclin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31722

Cat. No. GTX31722

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all Beclin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 - 2 μg/mL
ICC/IF Assay dependent
IHC-P 2 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

HEK293 Cell Lysate(GTX27902) , Mouse Brain Tissue Lysate(GTX27934)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Beclin-1 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human Beclin-1.The immunogen is located within amino acids 40 - 90 of Beclin-1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887625

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

beclin 1 , ATG6 , VPS30 , beclin1

Cellular Localization

Cytoplasm,Endosome membrane,Endoplasmic reticulum membrane,Endosome,Nucleus

Background

Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration (Zhong et al., 2009 [PubMed 19270693]).[supplied by OMIM, Jul 2010]

Database

Research Area

DATA IMAGES

Anti-Beclin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31722

GTX31722 IHC-P Image

IHC-P analysis of human brain tissue using GTX31722 Beclin 1 antibody.
Working concentration : 20 μg/ml

Anti-Beclin 1 antibody used in Western Blot (WB). GTX31722

GTX31722 WB Image

WB analysis of various samples using GTX31722 Beclin 1 antibody.
Dilution : 1 μg/ml
Laoding : 15 μg

Anti-Beclin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31722

GTX31722 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX31722 Beclin 1 antibody.
Working concentration : 20 μg/ml

Anti-Beclin 1 antibody used in Western Blot (WB). GTX31722

GTX31722 WB Image

WB analysis of various samples using GTX31722 Beclin 1 antibody.
Dilution : 1 μg/ml
Laoding : 15 μg

Anti-Beclin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31722

GTX31722 IHC-P Image

IHC-P analysis of human brain tissue using GTX31722 Beclin 1 antibody.
Working concentration : 2.5 μg/ml

Anti-Beclin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31722

GTX31722 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX31722 Beclin 1 antibody.
Dilution : 1 μg/ml
Antigen retrieval : heat mediation with a citrate buffer (pH6).

Anti-Beclin 1 antibody used in Western Blot (WB). GTX31722

GTX31722 WB Image

WB analysis of various samples using GTX31722 Beclin 1 antibody.
Dilution : 1 μg/ml
Laoding : 15 μg

Anti-Beclin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31722

GTX31722 IHC-P Image

IHC-P analysis of rat liver tissue using GTX31722 Beclin 1 antibody.
Dilution : 1 μg/ml
Antigen retrieval : heat mediation with a citrate buffer (pH6).

Anti-Beclin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31722

GTX31722 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX31722 Beclin 1 antibody.
Working concentration : 2 μg/ml

Anti-Beclin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31722

GTX31722 IHC-P Image

IHC-P analysis of rat brain tissue using GTX31722 Beclin 1 antibody.
Working concentration : 2 μg/ml

REFERENCE

Application Reference

Yu S et al. Sci Rep 2021; 11 (1) : 17523 Arsenic leads to autophagy of keratinocytes by increasing aquaporin 3 expression.
Application : WB
Reactivity : Human
Mohapatra S et al. Cell Death Differ 2020; (Epub) Estrogen and estrogen receptors chauffeur the sex-biased autophagic action in liver.
Application : WB
Tang YJ et al. Drug Des Devel Ther 2019; 13 : 513-521 Bisperoxovanadium protects against spinal cord injury by regulating autophagy via activation of ERK1/2 signaling.
Smirnov AV et al. Bull Exp Biol Med 2018; 165 (1) : 105-109 Expression of Beclin-1 and LAMP-2 in Rat Hippocampus under Conditions of Simulated Gravity Overload in the Caudocranial Vector.
Application : IHC-P
Reactivity : Rat
Lin S et al. Int J Biochem Cell Biol 2017; 87 : 54-68 endoplasmic reticulum-targeting photosensitizer hypericin confers chemo-sensitization towards * through inducing pro-death autophagy.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399