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Beclin 1 antibody

KO/KD
Anti-Beclin 1 antibody used in Immunoprecipitation (IP). GTX55535
Anti-Beclin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55535
Anti-Beclin 1 antibody used in Western Blot (WB). GTX55535
Anti-Beclin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX55535
Anti-Beclin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX55535
Anti-Beclin 1 antibody used in Western Blot (WB). GTX55535

Cat. No. GTX55535

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Beclin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
IP 1:50 - 1:100
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human BECN1 (NP_003757.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887929

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

beclin 1 , ATG6 , VPS30 , beclin1

Cellular Localization

Cytoplasm,Endosome membrane,Endoplasmic reticulum membrane,Endosome,Nucleus

Background

This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-Beclin 1 antibody used in Immunoprecipitation (IP). GTX55535

GTX55535 IP Image

IP analysis of HeLa cell lysate using GTX55535 Beclin 1 antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

Anti-Beclin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55535

GTX55535 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX55535 Beclin 1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-Beclin 1 antibody used in Western Blot (WB). GTX55535

GTX55535 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) 293T cell lysate using GTX55535 Beclin 1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Beclin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX55535

GTX55535 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX55535 Beclin 1 antibody.
Dilution : 1:100

Anti-Beclin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX55535

GTX55535 IHC-P Image

IHC-P analysis of human stomach tissue using GTX55535 Beclin 1 antibody.
Dilution : 1:100

Anti-Beclin 1 antibody used in Western Blot (WB). GTX55535

GTX55535 WB Image

WB analysis of various sample lysates using GTX55535 Beclin 1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

Application Reference

Hui-Ling Wang et al. Neural Regen Res 2021; 16 (6) : 1011-1016 Electroacupuncture improves learning and memory functions in a rat cerebral ischemia/reperfusion injury model through PI3K/Akt signaling pathway activation.
Application : WB
Reactivity : Rat
Picot S et al. Autophagy 2020; 16 (11) : 2017-2035 Identification of the autophagy pathway in a mollusk bivalve, Crassostrea gigas.
Koh EH et al. J Biol Chem 2016; 291 (47) : 24747-24755 Mitochondrial Activity in Human White Adipocytes Is Regulated by the Ubiquitin Carrier Protein 9/microRNA-30a Axis.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399