APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Mouse, Rat, Zebrafish, Bovine, Chicken, Pig, Japanese Medaka, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.96 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Ser90 of human Beclin 1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2887121
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
beclin 1 , ATG6 , VPS30 , beclin1
Cellular Localization
Cytoplasm,Endosome membrane,Endoplasmic reticulum membrane,Endosome,Nucleus
Background
This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Database
Research Area
DATA IMAGES
|
GTX133857 WB Image
Untreated (–) and treated (+) MCF-7 whole cell extracts (60 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Beclin1 (phospho Ser90) antibody (GTX133857) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
REFERENCE
There are currently no references for Beclin 1 (phospho Ser90) antibody (GTX133857). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Beclin 1 (phospho Ser90) antibody (GTX133857). Be the first to share your experience with this product.