Untreated (–) and treated (+) MCF-7 whole cell extracts (60 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Beclin1 (phospho Ser90) antibody (GTX133857) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.