ICC: Use at an assay dependent dilution. IHC: Use at an assay dependent dilution, GTX14928 stains weakly. IP: Use at a dilution of 1/200. GTX14928 immunoprecipitates poorly. WB: Use at a dilution of 1/500. Predicted molecular weight: 68 kDa. Optimal dilutions/concentrations should be determined by the end user.
Conditioned medium cultured RPE cells (the basal expression of Bestrophin is low in cultured cells).
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.75 mg/ml (Please refer to the vial label for the specific concentration.)
Synthetic peptide: WKLKAVDAFKSAPLYQRPGY, corresponding to amino acids 445-464 of Human Bestrophin. The peptide was amidated for conjugation.
Immunogen affinity purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
BEST, VMD2, Bestrophin, 7439, O76090, 153700, TU15B, BMD
Bestrophin is a 68 kDa basolateral plasma membrane protein encoded by the VMD2 gene. The role of Bestrophin is as yet unclear but it is thought to be involved in Best Macular Dystrophy (BMD) or Vitelliform Macular Dystrophy (VMD2); inherited early onset autosomal forms of macular degeneration. There is evidence to suggest that Bestrophin is a chloride channel that plays a role in generating the altered electrooculogram in Best disease patients.