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beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622]

Orthogonal
Anti-beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] used in ELISA (ELISA). GTX635160
Anti-beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] used in ELISA (ELISA). GTX635160
Anti-beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] used in Dot blot (Dot). GTX635160
Anti-beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] used in IHC (Paraffin sections) (IHC-P). GTX635160
Anti-beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] used in IHC (Paraffin sections) (IHC-P). GTX635160
Anti-beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] used in IHC (Paraffin sections) (IHC-P). GTX635160

Cat. No. GTX635160

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT622

Isotype

 IgG2b

Application

 IHC-P, Dot, ELISA, Sandwich ELISA

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all beta Amyloid products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:100-1:2000
Dot Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX635160, Detection: GTX134510
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

Predict Reactivity

Rat, Rabbit, Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.27 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The immunogen used to generate this antibody corresponds to Beta amyloid (1-42).

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888506

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

amyloid beta precursor protein , AAA , ABETA , ABPP , AD1 , APPI , CTFgamma , CVAP , PN-II , PN2 , preA4

Cellular Localization

Cell membrane,Membrane,Cytoplasmic vesicle,Cytoplasm

Background

This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. In addition, two of the peptides are antimicrobial peptides, having been shown to have bacteriocidal and antifungal activities. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Aug 2014]

Database

Research Area

DATA IMAGES

Anti-beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] used in ELISA (ELISA). GTX635160

GTX635160 ELISA Image

Sandwich ELISA detection of beta Amyloid (1-42)–peptide aggregate using GTX635160 as capture antibody at concentration of 10 μg/mL and GTX134510 as detection antibody at concentration of 0.45 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:2000 and used to detect the primary antibody.

Anti-beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] used in ELISA (ELISA). GTX635160

GTX635160 ELISA Image

Indirect ELISA analysis was performed by coating plate with 100 μL of beta Amyloid (1-42) peptide aggregate at concentration of 10 μg/mL. The coated protein is detected with beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] (GTX635160) at concentrations ranging from 3.06 to 2230 ng/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:2000 and used to detect the primary antibody.

Anti-beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] used in Dot blot (Dot). GTX635160

GTX635160 Dot Image

Dot blot analysis of 100 ng beta Amyloid 1-42 oligomers or monomers, using beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] (GTX635160) at 1:10000, 1:20000, 1:40000, and 1:80000 dilution.

Anti-beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] used in IHC (Paraffin sections) (IHC-P). GTX635160

GTX635160 IHC-P Image

Orthogonal

beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] detects beta Amyloid protein aggregates by immunohistochemical analysis in the hippocampus of 3xTg-AD transgenic mouse brain (left), but not in age- and gender- matched normal mouse brain (right).
Sample: Paraffin-embedded 3xTg-AD transgenic mouse brain (left) and healthy mouse brain (right).
Green: beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] (GTX635160) diluted at 1:2000.
Red: NeuN stained by NeuN antibody (GTX132974) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
This image was provided courtesy of a customer review.

Anti-beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] used in IHC (Paraffin sections) (IHC-P). GTX635160

GTX635160 IHC-P Image

Orthogonal

beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] detects beta amyloid protein aggregates by immunohistochemical analysis in the occipital lobe of Alzheimer's disease brain (left), but not in normal human brain(right).
Sample: Paraffin-embedded Human Brain (Alzheimer's Disease + Normal) tissue slides (GTX49382).
beta Amyloid protein aggregates stained by beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] (GTX635160) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] used in IHC (Paraffin sections) (IHC-P). GTX635160

GTX635160 IHC-P Image

Orthogonal

beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] detects beta Amyloid protein aggregates by immunohistochemical analysis in the occipital lobe of Alzheimer's disease brain (left), but not in normal human brain(right).
Sample: Paraffin-embedded Human Brain (Alzheimer's Disease + Normal) tissue slides (GTX49382).
beta Amyloid protein aggregates stained by beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] (GTX635160) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Bassil R et al. Nat Commun 2021; 12 (1) : 5220 Improved modeling of human AD with an automated culturing platform for iPSC neurons, astrocytes and microglia.
Application : ELISA
Reactivity : Human
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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169