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Beta-gal antibody

Anti-Beta-gal antibody used in Western Blot (WB). GTX132107
Anti-Beta-gal antibody used in Western Blot (WB). GTX132107

Cat. No. GTX132107

1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC

Reactivity

 E. coli
Package
100 μl ($399),
25 μl ($169)
See all Beta-gal products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
IHC Assay dependent
Not tested in other applications.

Calculated MW

116 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.05 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

E. coli

Immunogen

The immunogen used to generate this antibody corresponds to E. Coli beta-gal.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886568

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ECK0341 , JW0335

Database

Research Area

DATA IMAGES

Anti-Beta-gal antibody used in Western Blot (WB). GTX132107

GTX132107 WB Image

Various amounts of beta galactosidase protein were separated by 7.5% SDS-PAGE, and the membrane was blotted with Beta-gal antibody (GTX132107) diluted at 1:10000.

Anti-Beta-gal antibody used in Western Blot (WB). GTX132107

GTX132107 WB Image

Various amounts of beta galactosidase protein (120 ng | 30 ng μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Beta-gal antibody (GTX132107) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

Beta-gal antibody Cat. No. GTX132107

Rating
( Average 5 based on 1 users reviews)
Application
Immunohistochemistry(IHC)
( Average 5 based on 1 users reviews)
Anti-Beta-gal antibody used in Immunohistochemistry (IHC). GTX132107
Date : Anonymous submitted on 12-Sep-2017
Score :
Application Tested : IHC
Sample : Drosophila eye
Blocking : 5% goat serum (prepared with 0.3% Triton X-100)
Fixation : 4% paraformaldehyde in PBS
Permeabilization /Antigen retrieval : 0.3% Triton X-100
Primary Antibody : 1:100, 4°C, 16Hr
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169