GeneTex
United States (US)

Bid antibody [23F7]

GTX15667_281_ICC-IF_19011519_387.jpg
GTX15667_2070_WB_19011519_273.jpg
GTX15667_283_ICC-IF_19011519_770.jpg
GTX15667_284_ICC-IF_19011519_731.jpg
GTX15667_282_ICC-IF_19011519_434.jpg

Cat No. GTX15667

Host Mouse
Clonality Monoclonal
Clone Name 23F7
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
ICC/IF 1-2 μg/ml
Not tested in other applications.

Calculated MW

22 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Residues 5-195 from human recombinant protein expressed in bacteria

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Bh3 Interacting Domain Death Agonist,Fp497,Bid

Cellular Localization

Cytoplasm,Mitochondrion membrane

Background

This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX15667_281_ICC-IF_19011519_387.jpg

GTX15667 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX15667 Bid antibody [23F7]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Trito X-100 for 10 minutes
Dilution : 2 µg/ml in 0.1% BSA and incubated for 3 hours at room temperature

GTX15667_2070_WB_19011519_273.jpg

GTX15667 WB Image

WB analysis of 0.125-1ug purified BID-GST fusion protein using GTX15667 Bid antibody [23F7].
Dilution : 1:1000

GTX15667_283_ICC-IF_19011519_770.jpg

GTX15667 ICC/IF Image

ICC/IF analysis of Hela cells using GTX15667 Bid antibody [23F7]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
Dilution : 1:20 overnight at 4°C

GTX15667_284_ICC-IF_19011519_731.jpg

GTX15667 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX15667 Bid antibody [23F7]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
Dilution : 1:20 overnight at 4°C

GTX15667_282_ICC-IF_19011519_434.jpg

GTX15667 ICC/IF Image

ICC/IF analysis of A549 cells using GTX15667 Bid antibody [23F7]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
Dilution : 1:20 overnight at 4°C

Package List Price ($)
$ 319