ICC/IF analysis of NIH-3T3 cells using GTX15667 Bid antibody [23F7]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
Dilution : 1:20 overnight at 4ºC

ICC/IF analysis of A549 cells using GTX15667 Bid antibody [23F7]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
Dilution : 1:20 overnight at 4ºC

ICC/IF analysis of Hela cells using GTX15667 Bid antibody [23F7]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
Dilution : 1:20 overnight at 4ºC

ICC/IF analysis of HeLa cells using GTX15667 Bid antibody [23F7]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Trito X-100 for 10 minutes
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature

WB analysis of 0.125-1ug purified BID-GST fusion protein using GTX15667 Bid antibody [23F7].
Dilution : 1:1000