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Bid antibody [3C5]

Anti-Bid antibody [3C5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60429
Anti-Bid antibody [3C5] used in IHC (Paraffin sections) (IHC-P). GTX60429
Anti-Bid antibody [3C5] used in Western Blot (WB). GTX60429
Anti-Bid antibody [3C5] used in ELISA (ELISA). GTX60429
Anti-Bid antibody [3C5] used in Flow cytometry (FACS). GTX60429

Cat. No. GTX60429

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3C5

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all Bid products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human BID expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BH3 interacting domain death agonist , FP497

Cellular Localization

Cytoplasm,Mitochondrion outer membrane

Background

This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Bid antibody [3C5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60429

GTX60429 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60429 Bid antibody [3C5].
Green : Bid
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-Bid antibody [3C5] used in IHC (Paraffin sections) (IHC-P). GTX60429

GTX60429 IHC-P Image

IHC-P analysis of human prostate tissue (left) and tonsil tissue (right) using GTX60429 Bid antibody [3C5].

Anti-Bid antibody [3C5] used in Western Blot (WB). GTX60429

GTX60429 WB Image

WB analysis of HeLa (1), A431 (2), Jurkat (3), A549 (4), HepG2 (5), and HEK293 (6) cell lysate using GTX60429 Bid antibody [3C5].

Anti-Bid antibody [3C5] used in ELISA (ELISA). GTX60429

GTX60429 ELISA Image

ELISA analysis of antigen using GTX60429 Bid antibody [3C5].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

Anti-Bid antibody [3C5] used in Flow cytometry (FACS). GTX60429

GTX60429 FACS Image

FACS analysis of HeLa cells using GTX60429 Bid antibody [3C5].
Green : Bid
Purple : negative control

REFERENCE

Application Reference

Tung CL et al. Int J Med Sci 2022; 19 (10) : 1567-1575 Ivermectin induces cell cycle arrest and caspase-dependent apoptosis in human urothelial carcinoma cells.
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399