GeneTex

  • Country / Location Selection

United States (US)

Bid antibody [4D3]

Anti-Bid antibody [4D3] used in Western Blot (WB). GTX57542
Anti-Bid antibody [4D3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57542
Anti-Bid antibody [4D3] used in Western Blot (WB). GTX57542

Cat. No. GTX57542

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4D3

Isotype

 IgG1

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399)
See all Bid products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:2000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone 4D3 is derived from hybridization of mouse F2 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human BID protein.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BH3 interacting domain death agonist , BID , FP497 , Bid

Cellular Localization

Cytoplasm,Mitochondrion membrane

Background

This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Bid antibody [4D3] used in Western Blot (WB). GTX57542

GTX57542 WB Image

WB analysis of Jurkat whole cell lysate using GTX57542 Bid antibody.
Loading : 20 μg
Dilution : 1:1000

Anti-Bid antibody [4D3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57542

GTX57542 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX57542 Bid antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

Anti-Bid antibody [4D3] used in Western Blot (WB). GTX57542

GTX57542 WB Image

WB analysis of various samples using GTX57542 Bid antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : A549 whole cell lysate
Loading : 40 μg
Dilution : 1:1000

REFERENCE

There are currently no references for Bid antibody [4D3] (GTX57542). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for Bid antibody [4D3] (GTX57542). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399