GeneTex
United States (US)

Bid antibody [N1C3]

KO/KD IP/MS
GTX101323_39897_20180824_WB_shRNA_watermark_19011506_351.jpg
GTX101323_39897_IP_19011506_660.jpg
GTX101323_39897_IP_2_19011506_582.jpg
GTX101323_WB_19011506_426.jpg

Cat No. GTX101323

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2

Specificity/Sensitivity

IP/MS validation was supported by references (PMID:30377376)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Bid. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Bh3 Interacting Domain Death Agonist,Fp497,Bid

Cellular Localization

Cytoplasm , Mitochondrion membrane , BH3-interacting domain death agonist p15: Mitochondrion membrane , BH3-interacting domain death agonist p13: Mitochondrion membrane , Isoform 1: Cytoplasm , Isoform 3: Cytoplasm , Isoform 2: Mitochondrion membrane

Background

This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101323_39897_20180824_WB_shRNA_watermark_19011506_351.jpg

GTX101323 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (60 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bid antibody [N1C3] (GTX101323) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101323_39897_IP_19011506_660.jpg

GTX101323 IP Image

Bid antibody [N1C3] immunoprecipitates BID protein in IP experiments.
IP samples: Jurkat whole cell extract
A. 40 μg Jurkat whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of BID protein by 4 μg Bid antibody [N1C3] (GTX101323)
5 % SDS-PAGE
The immunoprecipitated BID protein was detected by Bid antibody [N1C3] (GTX101323) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

GTX101323_39897_IP_2_19011506_582.jpg

GTX101323 IP Image

Immunoprecipitation of Bid protein from Jurkat whole cell extracts using 5 μg of Bid antibody [N1C3] (GTX101323) or Bid antibody [N1C3-2] (GTX110568).
Western blot analysis was performed using Bid antibody [N1C3] (GTX101323).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX101323_WB_19011506_426.jpg

GTX101323 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
12% SDS PAGE
GTX101323 diluted at 1:1000

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169