Non-transfected (–) and transfected (+) 293T whole cell extracts (60 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bid antibody [N1C3] (GTX101323) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunoprecipitation of Bid protein from Jurkat whole cell extracts using 5 μg of Bid antibody [N1C3] (GTX101323) or Bid antibody [N1C3-2] (GTX110568).
Western blot analysis was performed using Bid antibody [N1C3] (GTX101323) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Bid antibody [N1C3] immunoprecipitates BID protein in IP experiments.
IP samples: Jurkat whole cell extract
A. 40 μg Jurkat whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of BID protein by 4 μg Bid antibody [N1C3] (GTX101323)
5 % SDS-PAGE
The immunoprecipitated BID protein was detected by Bid antibody [N1C3] (GTX101323) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
12% SDS PAGE
GTX101323 diluted at 1:1000