GeneTex
United States (US)

Bid antibody

Cat No. GTX10640

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

L929 cells treated with TNF-α
PROPERTIES

Form

Liquid

Buffer

PBS(without Mg2+ and Ca2+) pH7.3, 50% glycerol, 1.0 mg/ml BSA (IgG, protease free), 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Mouse

Immunogen

synthetic peptide corresponding to N-terminal of cleavage site [59/60] of mouse BID.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Bh3 Interacting Domain Death Agonist,2700049M22Rik,Ai875481,Au022477,Bid

Background

BH3 interacting domain death agonist (BID) is a pro-apoptotic member of the Bcl 2 family. BID interacts with both Bcl 2 and Bax through its BH3 domain. It usually exists in an inactive form in the cytosolic fraction of living cells and becomes cleaved and activated by caspase 8 in response to TNF alpha or Fas ligand. Once BID is cleaved, the C-terminal 15 kDa fragment of BID (p15) translocates onto mitochondria and is sufficient to trigger cytochrome c release, resulting in cell apoptosis. BID serves as a direct molecular link between caspase 8 activation and mitochondrial death machinery.

Database

Research Area

Package List Price ($)
$ 419