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Bid (p15-specific ) antibody

Anti-Bid (p15-specific ) antibody used in Western Blot (WB). GTX25498
Anti-Bid (p15-specific ) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25498

Cat. No. GTX25498

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
50 μl ($399)
See all Bid products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1:250
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Product Note

Recognizes the human C-terminal 15 kDa fragment of BID (p15).

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized peptide derived from the human BID fragment (p15).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_380561

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BH3 interacting domain death agonist , FP497

Cellular Localization

Cytoplasm,Mitochondrion outer membrane

Background

This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Bid (p15-specific ) antibody used in Western Blot (WB). GTX25498

GTX25498 WB Image

WB analysis of Jurkat cells without added BID (Lanes 1 and 2), with caspase-8 cleaved recombinant human BID (Lane 3), or with caspase-8 cleaved recombinant mouse BID (Lane 4) using either a human BID full length antibody (lane 1) or GTX25498 Bid (p15-specific ) antibody (lanes 2-4). The data show that the human BID (p15) antibody recognizes only the human 15 kDa BID fragment, demonstrating the specificity of the antibody.

Anti-Bid (p15-specific ) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25498

GTX25498 ICC/IF Image

ICC/IF analysis of A549 cells using GTX25498 Bid (p15-specific ) antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.25% Triton X-100 for 10 minutes
Dilution : 1:250 dilution in 1% BSA incubated for 3 hours at room temperature

REFERENCE

Application Reference

Li Y et al. Mol Cell Biochem 2009; 331 (43102) : 231-8 Alternative Fas-mediated cell death pathway is dependent on the different cleavage patterns of procaspase-8.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399