APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
0.3-1μg/ml |
2.5μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89961-PEP).
Predict Reactivity
Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS pH7.3, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-QKGKVPITYLELLN, from the C Terminus of the protein sequence according to NP_057093.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
RRID
AB_10732081
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
SH3 domain containing GRB2 like, endophilin B1 , Bif-1 , CGI-61 , PPP1R70 , dJ612B15.2
Cellular Localization
Cytoplasm , Golgi apparatus membrane , Mitochondrion outer membrane
Background
This gene encodes a SRC homology 3 domain-containing protein. The encoded protein interacts with the proapoptotic member of the Bcl-2 family, Bcl-2-associated X protein (Bax) and may be involved in regulating apoptotic signaling pathways. This protein may also be involved in maintaining mitochondrial morphology. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]
Database
Research Area
DATA IMAGES
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GTX89961 WB Image
WB analysis of Jurkat lysate using GTX89961 Bif-1 antibody, C-term. Dilution : 0.3μg/ml Loading : 35μg protein in RIPA buffer
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GTX89961 IHC-P Image
IHC-P analysis of human colon using GTX89961 Bif-1 antibody, C-term. Antigen retrieval : citrate buffer pH 6 Dilution : 2.5μg/ml
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REFERENCE
REVIEW
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