APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
NIH-3T3 , JC , BCL-1 , H1299
Predict Reactivity
Rat, Bovine, Chicken, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.57 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Bif-1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1951899
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
SH3 domain containing GRB2 like, endophilin B1 , Bif-1 , CGI-61 , PPP1R70 , dJ612B15.2
Cellular Localization
Cytoplasm , Golgi apparatus membrane; Peripheral membrane protein , Mitochondrion outer membrane; Peripheral membrane protein
Background
May be required for normal outer mitochondrial membrane dynamics. Required for coatomer-mediated retrograde transport in certain cells. May recruit other proteins to membranes with high curvature. May promote membrane fusion.
Database
Research Area
DATA IMAGES
![Anti-Bif-1 antibody [C2C3], C-term used in Western Blot (WB). GTX104636](/templates/default/js/lightbox/images/loading.gif "Anti-Bif-1 antibody [C2C3], C-term used in Western Blot (WB). GTX104636")
|
GTX104636 WB Image
Sample(30 μg of whole cell lysate)
A:H1299
7.5% SDS PAGE
GTX104636 diluted at 1:1000
|
|
|
GTX104636 WB Image
Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
10% SDS PAGE
GTX104636 diluted at 1:1000
|
|
|
GTX104636 ICC/IF Image
Bif-1 antibody [C2C3], C-term detects Bif-1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min.
Green: Bif-1 protein stained by Bif-1 antibody [C2C3], C-term (GTX104636) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm
|
|
|
GTX104636 IHC-P Image
Bif-1 antibody detects Bif-1 protein at cytosol on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
Bif-1 antibody (GTX104636) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
|
REFERENCE
There are currently no references for Bif-1 antibody [C2C3], C-term (GTX104636). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Bif-1 antibody [C2C3], C-term (GTX104636). Be the first to share your experience with this product.
![Anti-Bif-1 antibody [C2C3], C-term used in Western Blot (WB). GTX104636](/upload/website/prouct_img/small/GTX104636/GTX104636_WB_w_23060120_250.webp "Anti-Bif-1 antibody [C2C3], C-term used in Western Blot (WB). GTX104636")
![Anti-Bif-1 antibody [C2C3], C-term used in Western Blot (WB). GTX104636](/upload/website/prouct_img/small/GTX104636/GTX104636_39820_WB_M_w_23060120_457.webp "Anti-Bif-1 antibody [C2C3], C-term used in Western Blot (WB). GTX104636")
![Anti-Bif-1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104636](/upload/website/prouct_img/small/GTX104636/GTX104636_39820_20150723_IFA_w_23060120_553.webp "Anti-Bif-1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104636")
![Anti-Bif-1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104636](/upload/website/prouct_img/small/GTX104636/GTX104636_39820_IHC_w_23060120_202.webp "Anti-Bif-1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104636")
![Anti-Bif-1 antibody [C2C3], C-term used in Western Blot (WB). GTX104636](/upload/website/prouct_img/normal/GTX104636/GTX104636_WB_w_23060120_250.webp "Anti-Bif-1 antibody [C2C3], C-term used in Western Blot (WB). GTX104636")
![Anti-Bif-1 antibody [C2C3], C-term used in Western Blot (WB). GTX104636](/upload/website/prouct_img/normal/GTX104636/GTX104636_39820_WB_M_w_23060120_457.webp "Anti-Bif-1 antibody [C2C3], C-term used in Western Blot (WB). GTX104636")
![Anti-Bif-1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104636](/upload/website/prouct_img/normal/GTX104636/GTX104636_39820_20150723_IFA_w_23060120_553.webp "Anti-Bif-1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104636")
![Anti-Bif-1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104636](/upload/website/prouct_img/normal/GTX104636/GTX104636_39820_IHC_w_23060120_202.webp "Anti-Bif-1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104636")