GeneTex
United States (US)

Bif-1 antibody [C2C3], C-term

GTX104636_39820_WB_M_19011506_135.jpg
GTX104636_39820_20150723_IFA_19011506_595.jpg
GTX104636_39820_IHC_19011506_574.jpg
GTX104636_WB_19011506_713.jpg

Cat No. GTX104636

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

H1299 , NIH-3T3 , JC , BCL-1

Predict Reactivity

Rat, Chicken, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.57 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Bif-1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Sh3 Domain Containing Grb2 Like, Endophilin B1,Bif-1,Cgi-61,Ppp1R70,Dj612B15.2,Sh3Glb1

Cellular Localization

Cytoplasm , Golgi apparatus membrane; Peripheral membrane protein , Mitochondrion outer membrane; Peripheral membrane protein

Background

May be required for normal outer mitochondrial membrane dynamics. Required for coatomer-mediated retrograde transport in certain cells. May recruit other proteins to membranes with high curvature. May promote membrane fusion.

Database

Research Area

DATA IMAGES
GTX104636_39820_WB_M_19011506_135.jpg

GTX104636 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
10% SDS PAGE
GTX104636 diluted at 1:1000

GTX104636_39820_20150723_IFA_19011506_595.jpg

GTX104636 ICC/IF Image

Bif-1 antibody [C2C3], C-term detects Bif-1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min.
Green: Bif-1 protein stained by Bif-1 antibody [C2C3], C-term (GTX104636) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm

GTX104636_39820_IHC_19011506_574.jpg

GTX104636 IHC-P Image

Bif-1 antibody detects Bif-1 protein at cytosol on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
Bif-1 antibody (GTX104636) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104636_WB_19011506_713.jpg

GTX104636 WB Image

Sample(30 μg of whole cell lysate)
A:H1299
7.5% SDS PAGE
GTX104636 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169