GeneTex
United States (US)

Biglycan antibody

GTX102765_39918_IHC_19011506_550.jpg
GTX102765_WB_19011506_244.jpg

Cat No. GTX102765

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Mouse, Rat, Rabbit, Sheep, Bovine, Dog, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.27 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Biglycan. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Biglycan,Dspg1,Mrls,Pg-S1,Pgi,Semdx,Slrr1A,Bgn

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

The protein encoded by this gene is a small cellular or pericellular matrix proteoglycan that is closely related in structure to two other small proteoglycans, decorin and fibromodulin. The encoded protein and decorin are thought to be the result of a gene duplication. Decorin contains one attached glycosaminoglycan chain, while this protein probably contains two chains. For this reason, this protein is called biglycan. This protein is thought to function in connective tissue metabolism by binding to collagen fibrils and transfering growth factor-beta. It may promote neuronal survival. This gene is a candidate gene for the Happle syndrome. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102765_39918_IHC_19011506_550.jpg

GTX102765 IHC-P Image

Biglycan antibody detects BGN protein at cytosol on human Colon Epithelium by immunohistochemical analysis.
Sample: Paraffin-embedded Colon Epithelium.
Biglycan antibody (GTX102765) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102765_WB_19011506_244.jpg

GTX102765 WB Image

Sample(30 ug whole cell lysate)
A:293T
12% SDS PAGE
GTX102765 diluted at 1:500

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169