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Biglycan antibody

Anti-Biglycan antibody used in IHC (Paraffin sections) (IHC-P). GTX102765
Anti-Biglycan antibody used in Western Blot (WB). GTX102765

Cat. No. GTX102765

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Biglycan products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Mouse, Rat, Rabbit, Sheep, Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.27 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Biglycan. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949739

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

biglycan , DSPG1 , MRLS , PG-S1 , PGI , SEMDX , SLRR1A

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

The protein encoded by this gene is a small cellular or pericellular matrix proteoglycan that is closely related in structure to two other small proteoglycans, decorin and fibromodulin. The encoded protein and decorin are thought to be the result of a gene duplication. Decorin contains one attached glycosaminoglycan chain, while this protein probably contains two chains. For this reason, this protein is called biglycan. This protein is thought to function in connective tissue metabolism by binding to collagen fibrils and transfering growth factor-beta. It may promote neuronal survival. This gene is a candidate gene for the Happle syndrome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Biglycan antibody used in IHC (Paraffin sections) (IHC-P). GTX102765

GTX102765 IHC-P Image

Biglycan antibody detects BGN protein at cytosol on human Colon Epithelium by immunohistochemical analysis.
Sample: Paraffin-embedded Colon Epithelium.
Biglycan antibody (GTX102765) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Biglycan antibody used in Western Blot (WB). GTX102765

GTX102765 WB Image

Sample(30 ug whole cell lysate)
A:293T
12% SDS PAGE
GTX102765 diluted at 1:500

REFERENCE

Application Reference

Yoshizawa T et al. Glycobiology 2017; (Epub) Vascular abnormalities in the placenta of Chst14-/- fetuses: implications in the pathophysiology of perinatal lethality of the murine model and vascular lesions in human CHST14/D4ST1 deficiency.
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169