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Bim antibody [1C2C8]

Anti-Bim antibody [1C2C8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84981
Anti-Bim antibody [1C2C8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84981
Anti-Bim antibody [1C2C8] used in Western Blot (WB). GTX84981

Cat. No. GTX84981

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1C2C8

Isotype

 IgG1

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($429)
See all Bim products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL
ICC/IF 10 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

K-562 Cell Slide(GTX25535) , Bim Recombinant Protein

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Mouse monoclonal Bim antibody was raised against a recombinant protein corresponding to amino acids 2 to 198 of human Bim.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCL2 like 11 , BAM , BIM , BOD

Background

Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. Bim is another BH3 domain containing protein which can induce apoptosis. Bim interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines.

Database

Research Area

DATA IMAGES

Anti-Bim antibody [1C2C8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84981

GTX84981 ICC/IF Image

ICC/IF analysis of K562 cells using GTX84981 Bim antibody [1C2C8].
Working concentration : 20 μg/ml

Anti-Bim antibody [1C2C8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84981

GTX84981 ICC/IF Image

ICC/IF analysis of K562 cells using GTX84981 Bim antibody [1C2C8].
Working concentration : 10 μg/ml

Anti-Bim antibody [1C2C8] used in Western Blot (WB). GTX84981

GTX84981 WB Image

WB analysis of (A) 5 and (B) 25 ng Bim recombinant protein using GTX84981 Bim antibody [1C2C8].
Working concentration : 1 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 429