GeneTex
United States (US)

Bim antibody, C-term

GTX17031_1505_WB_18121410_159.jpg

Cat No. GTX17031

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-2μg/ml
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX17031-PEP).

Specificity/Sensitivity

This antibody is expected to recognise at least reported isoforms Bim-alpha1 (BimABCD), Bim-alpha2 (BimACD) and Bim-alpha3 (BimAD).
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-FNAYYARRLEK, from the C Terminus of the protein sequence according to NP_619528.1; NP_619529.1; NP_996886.1;.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Bcl2 Like 11,Bam,Bim,Bod,Bcl2L11

Cellular Localization

Intracytoplasmic membranes

Background

The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The protein encoded by this gene contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the BCL-2 protein family, including BCL2, BCL2L1/BCL-X(L), and MCL1, and to act as an apoptotic activator. The expression of this gene can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role of this gene in neuronal and lymphocyte apoptosis. Transgenic studies of the mouse counterpart suggested that this gene functions as an essential initiator of apoptosis in thymocyte-negative selection. Several alternatively spliced transcript variants of this gene have been identified. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX17031_1505_WB_18121410_159.jpg

GTX17031 WB Image

WB analysis of K562 lysate using GTX17031 Bim antibody, C-term.
Dilution : 0.5µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329