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Bim antibody [GT1234]

Recombinant antibody
Anti-Bim antibody [GT1234] used in Western Blot (WB). GTX01542
Anti-Bim antibody [GT1234] used in Western Blot (WB). GTX01542
Anti-Bim antibody [GT1234] used in Western Blot (WB). GTX01542

Cat. No. GTX01542

Reference 1

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1234

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($419)
See all Bim products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human Bim.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BAM,BCL2 like 11,BCL2L11,BIM,BOD,Bim

Cellular Localization

Endomembrane system,Mitochondrion

Background

The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The protein encoded by this gene contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the BCL-2 protein family and to act as an apoptotic activator. The expression of this gene can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role of this gene in neuronal and lymphocyte apoptosis. Transgenic studies of the mouse counterpart suggested that this gene functions as an essential initiator of apoptosis in thymocyte-negative selection. Several alternatively spliced transcript variants of this gene have been identified. [provided by RefSeq, Jun 2013]

Database

Research Area

DATA IMAGES

Anti-Bim antibody [GT1234] used in Western Blot (WB). GTX01542

GTX01542 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Bim antibody [GT1234] (GTX01542) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Multiple bands were observed 25 kDa, 15 kDa, and 10 kDa. It is possibly due to alternative splicing.
Reference: PMID: 29358700

Anti-Bim antibody [GT1234] used in Western Blot (WB). GTX01542

GTX01542 WB Image

WB analysis of various samples using GTX01542 Bim antibody [GT1234].
Dilution : 1:1000
Loading : 25 μg

Anti-Bim antibody [GT1234] used in Western Blot (WB). GTX01542

GTX01542 WB Image

WB analysis of various samples using GTX01542 Bim antibody [GT1234].
Dilution : 1:1000
Loading : 25 μg

REFERENCE

Application Reference

G Mendez-Callejas et al. Plants 2024; 13 (5) : 570 Natural 2′,4-Dihydroxy-4′,6′-dimethoxy Chalcone Isolated from Chromolaena tacotana Inhibits Breast Cancer Cell Growth through Autophagy and Mitochondrial Apoptosis
Application : WB
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 419