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Bim antibody, Internal

Anti-Bim antibody, Internal used in Western Blot (WB). GTX81177
Anti-Bim antibody, Internal used in Flow cytometry (FACS). GTX81177
Anti-Bim antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX81177
Anti-Bim antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX81177

Cat. No. GTX81177

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS

Reactivity

 Human
Package
400 μl ($399)
See all Bim products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P 1:10-1:50
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 134-160 amino acids from the Central region of human BCL2L11.

Purification

Protein A purified, followed by peptide affinity purification.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCL2 like 11 , BAM , BIM , BOD

Background

The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The protein encoded by this gene contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the BCL-2 protein family and to act as an apoptotic activator. The expression of this gene can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role of this gene in neuronal and lymphocyte apoptosis. Transgenic studies of the mouse counterpart suggested that this gene functions as an essential initiator of apoptosis in thymocyte-negative selection. Several alternatively spliced transcript variants of this gene have been identified. [provided by RefSeq, Jun 2013]

Database

Research Area

DATA IMAGES

Anti-Bim antibody, Internal used in Western Blot (WB). GTX81177

GTX81177 WB Image

WB analysis of K562, HL-60 cell lysate (35ug/lane) using GTX81177 Bim antibody, Internal.

Anti-Bim antibody, Internal used in Flow cytometry (FACS). GTX81177

GTX81177 FACS Image

FACS analysis of WiDr cells using GTX81177 Bim antibody, Internal.
Top histogram : negative control
Bottom histogram : WiDr cells

Anti-Bim antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX81177

GTX81177 IHC-P Image

IHC-P analysis of human kidney tissue using GTX81177 Bim antibody, Internal.
Dilution : 1:25

Anti-Bim antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX81177

GTX81177 IHC-P Image

IHC-P analysis of human colon carcinoma using GTX81177 Bim antibody, Internal.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399