GeneTex
United States (US)

Bim antibody

Bim-antibody_IHC_GTX27888-1_18121409_895.jpg
Bim-antibody_WB_GTX27888-1_18121409_886.jpg

Cat No. GTX27888

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

For WB: Use at a dilution of 1:900 - 1:1,100 dilution. Optimal concentrations/dilutions should be determined by the researcher.

Calculated MW

22 kDa. ( Note )

Positive Control

Human K562 cell lysate
PROPERTIES

Form

Liquid

Buffer

PBS, pH 7.4 with 0.02% sodium azide as preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide corresponding to amino acids 22 to 40 of human origin (1). The sequence is identical to that of mouse anddiffers from that of rat by one amino acid.

Purification

Affinity purified with antigen

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Bcl2 Like 11,Bam,Bim,Bod,Bcl2L11

Cellular Localization

Intracytoplasmic membranes

Background

Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2homology 3 (BH3) domain is a potent death domain. BH3domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2family. A novel BH3 domain containing protein was recently identified and designated Bim or BOD in human, mouse and rat (1,2). Bim/BOD interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. Bim/BOD induces apoptosis. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines(1,2).

Database

Research Area

DATA IMAGES
Bim-antibody_IHC_GTX27888-1_18121409_895.jpg

GTX27888 IHC Image

Immunohistochemistry of Bim in human skin cancer cells with Bim Antibody (Cat. No. GTX27888) at 20 μg/mL.

Bim-antibody_WB_GTX27888-1_18121409_886.jpg

GTX27888 WB Image

Western blot analysis of Bim expression in human K562 (K) and A549 (A) cell lysates with Anti-Bim (Cat. No. GTX27888) at 2 μg/mL.

Package List Price ($)
$ 359