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Bim antibody

Anti-Bim antibody used in Western Blot (WB). GTX31697
Anti-Bim antibody used in Western Blot (WB). GTX31697
Anti-Bim antibody used in Western Blot (WB). GTX31697
Anti-Bim antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31697
Anti-Bim antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31697

Cat. No. GTX31697

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all Bim products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 5 μg/mL
ICC/IF 10 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

K-562 Cell Slide(GTX25535) , K562 Cell Lysate(GTX27911)

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Bim antibody was raised against a peptide corresponding to amino acids near the center of human Bim.The sequence is identical to that of mouse and rat.The immunogen is located within amino acids 80 - 130 of Bim.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCL2 like 11 , BAM , BIM , BOD

Background

The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The protein encoded by this gene contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the BCL-2 protein family, including BCL2, BCL2L1/BCL-X(L), and MCL1, and to act as an apoptotic activator. The expression of this gene can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role of this gene in neuronal and lymphocyte apoptosis. Transgenic studies of the mouse counterpart suggested that this gene functions as an essential initiator of apoptosis in thymocyte-negative selection. Several alternatively spliced transcript variants of this gene have been identified. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Bim antibody used in Western Blot (WB). GTX31697

GTX31697 WB Image

WB analysis of various cell lines using GTX31697 Bim antibody.
Dilution : 5μg/mL
Loading : 15μg of lysates per lane

Anti-Bim antibody used in Western Blot (WB). GTX31697

GTX31697 WB Image

WB analysis of Daudi and HeLa cell lysate using GTX31697 Bim antibody.
Dilution : 5μg/mL
Loading : 15μg of lysates per lane

Anti-Bim antibody used in Western Blot (WB). GTX31697

GTX31697 WB Image

WB analysis of various samples using GTX31697 Bim antibody.
Lane 1 : Human urinary bladder tissue lysate
Lane 2 : Human pancreas tissue lysate
Dilution : 5μg/mL
Loading : 15μg of lysates per lane

Anti-Bim antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31697

GTX31697 ICC/IF Image

ICC/IF analysis of formaldehyde-fixed K562 cells using GTX31697 Bim antibody.
Antigen retrieval : Heat mediation with a citrate buffer (pH6)
Dilution : 10μg/mL

Anti-Bim antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31697

GTX31697 ICC/IF Image

ICC/IF analysis of 4% paraformaldehyde-fixed K562 cells using GTX31697 Bim antibody.
Dilution : 20μg/mL

REFERENCE

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399