GeneTex
United States (US)

Bim antibody

GTX31697_287_ICC-IF_20180221_18121410_728.jpg
GTX31697_286_ICC-IF_20180221_18121410_338.jpg
GTX31697_2863_WB_20180221_18121410_295.jpg

Cat No. GTX31697

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 2.5 to 5 μg/mL
ICC/IF 10 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

K-562 Cell Slide(GTX25535) , K562 Cell Lysate(GTX27911)
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Bim antibody was raised against a peptide corresponding to amino acids near the center of human Bim.The sequence is identical to that of mouse and rat.The immunogen is located within amino acids 80 - 130 of Bim.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Bcl2 Like 11,Bam,Bim,Bod,Bcl2L11

Background

The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The protein encoded by this gene contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the BCL-2 protein family, including BCL2, BCL2L1/BCL-X(L), and MCL1, and to act as an apoptotic activator. The expression of this gene can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role of this gene in neuronal and lymphocyte apoptosis. Transgenic studies of the mouse counterpart suggested that this gene functions as an essential initiator of apoptosis in thymocyte-negative selection. Several alternatively spliced transcript variants of this gene have been identified. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX31697_287_ICC-IF_20180221_18121410_728.jpg

GTX31697 ICC/IF Image

ICC/IF analysis of K562 cells using GTX31697 Bim antibody.
Working concentration : 20 μg/ml

GTX31697_286_ICC-IF_20180221_18121410_338.jpg

GTX31697 ICC/IF Image

ICC/IF analysis of K562 cells using GTX31697 Bim antibody.
Working concentration : 10 μg/ml

GTX31697_2863_WB_20180221_18121410_295.jpg

GTX31697 WB Image

WB analysis of K562 cell lysate using GTX31697 Bim antibody.
Working concentration : (A) 2.5 and (B) 5 μg/ml

Package List Price ($)
$ 329