GeneTex

  • Country / Location Selection

United States (US)

Bim antibody

Orthogonal
Anti-Bim antibody used in Western Blot (WB). GTX50597
Anti-Bim antibody used in IHC (Paraffin sections) (IHC-P). GTX50597

Cat. No. GTX50597

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all Bim products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Product Note

It should recognize BIM isoform 1, isoform2, isoform4, isoform7, isoform13, and isoform 14 based on sequence homology.

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around aa.67~71 (P-A-S-P-G) derived from human Bim.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCL2 like 11 , BAM , BIM , BOD

Background

The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The protein encoded by this gene contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the BCL-2 protein family and to act as an apoptotic activator. The expression of this gene can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role of this gene in neuronal and lymphocyte apoptosis. Transgenic studies of the mouse counterpart suggested that this gene functions as an essential initiator of apoptosis in thymocyte-negative selection. Several alternatively spliced transcript variants of this gene have been identified. [provided by RefSeq, Jun 2013]

Database

Research Area

DATA IMAGES

Anti-Bim antibody used in Western Blot (WB). GTX50597

GTX50597 WB Image

Orthogonal

WB analysis of extracts from HeLa cells using GTX50597 Bim antibody.

Anti-Bim antibody used in IHC (Paraffin sections) (IHC-P). GTX50597

GTX50597 IHC-P Image

Orthogonal

IHC-P analysis of human breast carcinoma tissue using GTX50597 Bim antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

There are currently no references for Bim antibody (GTX50597). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for Bim antibody (GTX50597). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399