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United States (US)

Biotin antibody (AP)

Cat No. GTX26652

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA, IHC
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2500
ELISA 1:3000
IHC 1:200 - 1:1000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 and 0.1% (w/v) Sodium Azide and 10 mg/mL Bovine Serum Albumin (BSA)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)

Purification

Purified by antigen-affinity chromatography.
From polyclonal serum

Conjugation

Alkaline Phosphatase (AP)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Background

Affinity purified polyclonal anti-Biotin can be used to detect both biotinylated proteins and nucleic acids

Research Area

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339