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Biotin antibody (TxRd)

Anti-Biotin antibody (TxRd) used in Western Blot (WB). GTX26653

Cat. No. GTX26653

Reference 1

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, Dot, ELISA

Reactivity

 Species independent
Package
100 μg ($399)
See all Biotin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1:500-1:2500
Dot Assay dependent
ELISA Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl, 1% BSA

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Biotin conjugated to -KLH

Purification

Purified by antigen-affinity chromatography.
From serum

Conjugation

Texas Red (TxRd)
Ratio : 1.1 molecules Texas Red per Goat IgG molecule.

RRID

AB_385979

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Affinity purified polyclonal anti-Biotin can be used to detect both biotinylated proteins and nucleic acids.

Research Area

DATA IMAGES

Anti-Biotin antibody (TxRd) used in Western Blot (WB). GTX26653

GTX26653 WB Image

WB analysis of biotin-conjugated pig IgG using GTX26653 Biotin antibody (TxRd).
Loading : 50 ng
Dilution : 1:1000

REFERENCE

Application Reference

Genoveva Morral-Ruiz et al. Nano Research 2014; Biotinylated Polyurethane-urea Nanoparticles for Targeted Theranostics in Human Hepatocellular Carcinoma
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399