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Biotin antibody

Anti-Biotin antibody used in Western Blot (WB). GTX124262

Cat. No. GTX124262

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Species independent
Package
100 μl ($399),
25 μl ($169)
See all Biotin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:90000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to Biotin

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11174491

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Research Area

DATA IMAGES

Anti-Biotin antibody used in Western Blot (WB). GTX124262

GTX124262 WB Image

Western blot experiment using Biotin Antibody (GTX124262) at 1:90000 dilution to detect 60ng of biotinylated protein and non-biotinylated control.
A: Non-biotinlated control.
B: Biotinylated protein.

REFERENCE

Application Reference

Wang SA et al. Cell Death Differ 2021; (Epub) USP24 promotes drug resistance during cancer therapy.
Application : WB
Heit C et al. Free Radic Biol Med 2016; Catalase Deletion Promotes PreDiabetic Phenotype in Mice.
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169